VCAM1 antibody (FITC)

Catalog No. ABIN7835596

The Rat Monoclonal anti-VCAM1 antibody is suitable to detect VCAM1 in samples from Mouse. It has been validated for FACS and IF.

Quick Overview for VCAM1 antibody (FITC) (ABIN7835596)

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This VCAM1 antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: M-K-2

Product Details anti-VCAM1 Antibody (FITC)

Purpose: Rat monoclonal antibody FITC labled to CD106

Specificity: Recognizes mouse CD106

Purification: The antibody was purified by affinity chromatography.

Immunogen: Mouse preadipose cell line PA6

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for VCAM1

Target: VCAM1 (Vascular Cell Adhesion Molecule 1 (VCAM1))

Alternative Name: VCAM1

Background: L1CAM, Vascular cell adhesion protein 1, V-CAM 1, VCAM-1, INCAM-100, CD106

Gene ID: 22329

Pathways: Carbohydrate Homeostasis