LAMP2 antibody (Alexa Fluor 488)

Catalog No. ABIN7835613

This Mouse Monoclonal antibody specifically detects LAMP2 in FACS and IF. It exhibits reactivity toward Human.

Quick Overview for LAMP2 antibody (Alexa Fluor 488) (ABIN7835613)

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This LAMP2 antibody is conjugated to Alexa Fluor 488

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: H4B4

Product Details anti-LAMP2 Antibody (Alexa Fluor 488)

Purpose: Mouse monoclonal antibody AF488 labled to CD107b

Specificity: Recognizes human CD107b

Purification: The antibody was purified by affinity chromatography.

Immunogen: Adult human adherent spleen cells

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for LAMP2

Target: LAMP2 (Lysosomal-Associated Membrane Protein 2 (LAMP2))

Alternative Name: LAMP2

Background: Lysosome-associated membrane glycoprotein 2, LAMP-2, Lysosome-associated membrane protein 2, CD107 antigen-like family member B, CD107b

Gene ID: 3920

UniProt: P13473

Pathways: Autophagy