IL3RA antibody (APC)

Catalog No. ABIN7835701

The Mouse Monoclonal anti-IL3RA antibody is suitable to detect IL3RA in samples from Human. It has been validated for FACS and IF.

Quick Overview for IL3RA antibody (APC) (ABIN7835701)

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This IL3RA antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 6H6

Product Details anti-IL3RA Antibody (APC)

Purpose: Mouse monoclonal antibody APC labled to CD123

Specificity: Recognizes human CD123

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD123.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for IL3RA

Target: IL3RA (Interleukin 3 Receptor, alpha (IL3RA))

Alternative Name: IL3RA

Background: IL3R, Interleukin-3 receptor subunit alpha, IL-3 receptor subunit alpha, IL-3R subunit alpha, IL-3R-alpha, IL-3RA, CD antigen CD123

Gene ID: 3563

UniProt: P26951

Pathways: JAK-STAT Signaling