CCR5 antibody

Catalog No. ABIN7835902

The Rat Monoclonal anti-CCR5 antibody is suitable to detect CCR5 in samples from Human. It has been validated for FACS and IF.

Quick Overview for CCR5 antibody (ABIN7835902)

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This CCR5 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: HEK-1-85a

Product Details anti-CCR5 Antibody

Purpose: Rat monoclonal antibody to CD195

Specificity: Recognizes human CD195

Purification: The antibody was purified by affinity chromatography.

Immunogen: CHO cells transfected with human CCR5

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for CCR5

Target: CCR5 (Chemokine (C-C Motif) Receptor 5 (CCR5))

Alternative Name: CCR5

Background: CMKBR5, C-C chemokine receptor type 5, C-C CKR-5, CC-CKR-5, CCR-5, CCR5, CHEMR13, HIV-1 fusion coreceptor, CD195

Gene ID: 1234

UniProt: P51681

Pathways: Cellular Response to Molecule of Bacterial Origin, cAMP Metabolic Process, Regulation of Cell Size