LAG3 antibody

Catalog No. ABIN7835970

This Rat Monoclonal antibody specifically detects LAG3 in FACS and IF. It exhibits reactivity toward Mouse.

Quick Overview for LAG3 antibody (ABIN7835970)

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This LAG3 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: C9B7W

Product Details anti-LAG3 Antibody

Purpose: Rat monoclonal antibody to CD223

Specificity: Recognizes mouse CD223

Purification: The antibody was purified by affinity chromatography.

Immunogen: Murine CD223-Ig fusion protein

Application Details

Application Notes: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-rat IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for LAG3

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Alternative Name: LAG3

Background: FDC, Lymphocyte activation gene 3 protein, LAG-3, Protein FDC, CD223

Gene ID: 16768

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints