JAK2 antibody (pTyr1007, pTyr1008) (Cy5.5)

Catalog No. ABIN740960

The Rabbit Polyclonal anti-JAK2 antibody has been validated for WB, FACS, IF (cc) and IF (p). It is suitable to detect JAK2 in samples from Human, Rat, Mouse, Rabbit and Zebrafish (Danio rerio).

Quick Overview for JAK2 antibody (pTyr1007, pTyr1008) (Cy5.5) (ABIN740960)

Target: JAK2 (Janus Kinase 2 (JAK2))

Reactivity: Human, Rat, Mouse, Rabbit, Zebrafish (Danio rerio)

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JAK2 antibody is conjugated to Cy5.5

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p))

Product Details anti-JAK2 Antibody (Cy5.5)

Binding Specificity: pTyr1007, pTyr1008

Cross-Reactivity: Human, Mouse, Rabbit, Rat, Zebrafish (Danio rerio)

Predicted Reactivity: Pig,Chicken

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from human JAK2 around the phosphorylation site of Tyr1007/1008

Isotype: IgG

Application Details

Application Notes: FCM 1:20-100
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: Aqueous buffered solution containing 0.01M TBS ( pH 7.4) with 1 % BSA, 0.03 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at -20°C. Aliquot into multiple vials to avoid repeated freeze-thaw cycles.

Expiry Date: 12 months

Target Details for JAK2

Target: JAK2 (Janus Kinase 2 (JAK2))

Alternative Name: Jak2

Background:

Synonyms: JAK2Tyr1007/1008, Tyrosine protein kinase JAK2, JAK 2, JAK-2, JAK2, JAK2_HUMAN, Janus Activating Kinase 2, Janus Kinase 2, JTK 10, JTK10, OTTHUMP00000043260, Tyrosine-protein kinase JAK2, Tyrosine protein kinase JAK2.

Background: JAK2 (Janus Activating Kinase 2) is a tyrosine kinase of the non-receptor type, that associates with the intracellular domains of cytokine receptors, JAK2 is the predominant JAK kinase activated in response to several growth factors and cytokines such as IL-3, GM-CSF and erythropoietin, it has been found to be constitutively associated with the prolactin receptor and is required for responses to gamma interferon. Ligand binding to a variety of cell surface receptors (e.g., cytokine, growth factor, GPCRs) leads to an association of those receptors with JAK proteins, which are then activated via phosphorylation on tyrosines 1007 and 1008 in the kinase activation loop. Activated JAK proteins phosphorylate and activate STAT (signal transducers and activators of transcription) proteins, which then dimerize and translocate to the nucleus. Once in the nucleus, STAT proteins bind to DNA and modify the transcription of various genes.

Gene ID: 3717

UniProt: O60674

Pathways: JAK-STAT Signaling, RTK Signaling, Interferon-gamma Pathway, Positive Regulation of Peptide Hormone Secretion, Intracellular Steroid Hormone Receptor Signaling Pathway, Response to Growth Hormone Stimulus, Positive Regulation of Endopeptidase Activity, Protein targeting to Nucleus, CXCR4-mediated Signaling Events, Platelet-derived growth Factor Receptor Signaling, Unfolded Protein Response