CD24 antibody (APC)

Catalog No. ABIN7835992

The Rat Monoclonal anti-CD24 antibody is suitable to detect CD24 in samples from Mouse. It has been validated for FACS and IF.

Quick Overview for CD24 antibody (APC) (ABIN7835992)

Target: CD24 (CD24 Molecule (CD24))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This CD24 antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 30-F1

Product Details anti-CD24 Antibody (APC)

Purpose: Rat monoclonal antibody APC labled to CD24

Specificity: Recognizes mouse CD24

Purification: The antibody was purified by affinity chromatography.

Immunogen: C57BL/10 mouse splenic T cells and concanavalin A-activated splenocytes

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for CD24

Target: CD24 (CD24 Molecule (CD24))

Alternative Name: CD24

Background: CD24A, Signal transducer CD24, Small cell lung carcinoma cluster 4 antigen, CD24

Gene ID: 12484

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation