ICOS antibody

Catalog No. ABIN7836054

This Armenian Hamster Monoclonal antibody specifically detects ICOS in FACS and IF. It exhibits reactivity toward Human, Mouse and Rat.

Quick Overview for ICOS antibody (ABIN7836054)

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Reactivity: Human, Mouse, Rat

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This ICOS antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: C398-4A

Product Details anti-ICOS Antibody

Purpose: Armenian Hamster monoclonal antibody to CD278

Specificity: Recognizes human/mouse/rat CD278

Purification: The antibody was purified by affinity chromatography.

Immunogen: Mouse T cell clone D10.G4.1

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for ICOS

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Alternative Name: ICOS

Background: AILIM, Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, CD278

Gene ID: 29851, 54167, 64545

UniProt: Q9Y6W8

Pathways: Cancer Immune Checkpoints