ICOS antibody (APC)

Catalog No. ABIN7836055

The Armenian Hamster Monoclonal anti-ICOS antibody is suitable to detect ICOS in samples from Human. It has been validated for FACS and IF.

Quick Overview for ICOS antibody (APC) (ABIN7836055)

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Reactivity: Human

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This ICOS antibody is conjugated to APC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: C398-4A

Product Details anti-ICOS Antibody (APC)

Purpose: Armenian Hamster monoclonal antibody APC labled to CD278

Specificity: Recognizes human CD278

Purification: The antibody was purified by affinity chromatography.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for ICOS

Target: ICOS (Inducible T-Cell Co-Stimulator (ICOS))

Alternative Name: ICOS

Background: AILIM, Inducible T-cell costimulator, Activation-inducible lymphocyte immunomediatory molecule, CD278

Gene ID: 29851

UniProt: Q9Y6W8

Pathways: Cancer Immune Checkpoints