BST2 antibody

Catalog No. ABIN7836143

The Rat Monoclonal anti-BST2 antibody (Clone 927) (ABIN7836143) specifically detects BST2 in FACS and IF. The antibody is reactive with Mouse samples.

Quick Overview for BST2 antibody (ABIN7836143)

Target: BST2 (Bone Marrow Stromal Cell Antigen 2 (BST2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This BST2 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 927

Product Details anti-BST2 Antibody

Purpose: Rat monoclonal antibody to CD317

Specificity: Recognizes mouse CD317

Purification: The antibody was purified by affinity chromatography.

Immunogen: Mouse plasmacytoid dendritic cells (DCs)

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for BST2

Target: BST2 (Bone Marrow Stromal Cell Antigen 2 (BST2))

Alternative Name: BST2

Background: Bone marrow stromal antigen 2, BST-2, HM1.24 antigen, Tetherin, CD317

Gene ID: 69550

Pathways: Regulation of Leukocyte Mediated Immunity, Production of Molecular Mediator of Immune Response