CD37 antibody (PE)

Catalog No. ABIN7836222

This Mouse Monoclonal antibody specifically detects CD37 in FACS and IF. It exhibits reactivity toward Human.

Quick Overview for CD37 antibody (PE) (ABIN7836222)

Target: CD37 (TSPAN26) (Tetraspanin 26 (TSPAN26))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD37 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: IPO-24

Product Details anti-CD37 Antibody (PE)

Purpose: Mouse monoclonal antibody PE labled to CD37

Specificity: Recognizes human CD37

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD37.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for CD37

Target: CD37 (TSPAN26) (Tetraspanin 26 (TSPAN26))

Alternative Name: CD37

Background: TSPAN26, Leukocyte antigen CD37, Tetraspanin-26, Tspan-26, CD antigen CD37

Gene ID: 951

UniProt: P11049

Pathways: Positive Regulation of Immune Effector Process, Production of Molecular Mediator of Immune Response