GP1BB antibody (PE-Cy5)

Catalog No. ABIN7836304

The Mouse Monoclonal anti-GP1BB antibody is suitable to detect GP1BB in samples from Human. It has been validated for IF and FACS.

Quick Overview for GP1BB antibody (PE-Cy5) (ABIN7836304)

Target: GP1BB (Glycoprotein Ib (Platelet), beta Polypeptide (GP1BB))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This GP1BB antibody is conjugated to PE-Cy5

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: MM2-174

Product Details anti-GP1BB Antibody (PE-Cy5)

Purpose: Mouse monoclonal antibody PE/Cy5 labled to CD42b

Specificity: Recognizes human CD42b

Purification: The antibody was purified by affinity chromatography.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for GP1BB

Target: GP1BB (Glycoprotein Ib (Platelet), beta Polypeptide (GP1BB))

Alternative Name: GP1BB

Background: Platelet glycoprotein Ib beta chain, GP-Ib beta, GPIb-beta, GPIbB, Antigen CD42b-beta, CD42c

Gene ID: 2812

UniProt: P13224