ITGA2 antibody (PE)

Catalog No. ABIN7836409

The Rat Monoclonal anti-ITGA2 antibody is suitable to detect ITGA2 in samples from Mouse. It has been validated for FACS and IF.

Quick Overview for ITGA2 antibody (PE) (ABIN7836409)

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This ITGA2 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: DX5

Product Details anti-ITGA2 Antibody (PE)

Purpose: Rat monoclonal antibody APC labled to CD49b

Specificity: Recognizes mouse CD49b

Purification: The antibody was purified by affinity chromatography.

Immunogen: Mouse colon carcinoma cell line Colon26

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for ITGA2

Target: ITGA2 (Integrin alpha 2 (ITGA2))

Alternative Name: ITGA2

Background: CD49B, Integrin alpha-2, CD49 antigen-like family member B, Collagen receptor, Platelet membrane glycoprotein Ia, GPIa, VLA-2 subunit alpha, CD49b

Gene ID: 16398

Pathways: CXCR4-mediated Signaling Events, Smooth Muscle Cell Migration, Integrin Complex