Selectin E/CD62e antibody

Catalog No. ABIN7836501

This Mouse Monoclonal antibody specifically detects Selectin E/CD62e in FACS and IF. It exhibits reactivity toward Human.

Quick Overview for Selectin E/CD62e antibody (ABIN7836501)

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Selectin E/CD62e antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: CL2

Product Details anti-Selectin E/CD62e Antibody

Purpose: Mouse monoclonal antibody to CD62E

Specificity: Recognizes human CD62E

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD62E

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Alternative Name: SELE

Background: ELAM1, E-selectin, CD62 antigen-like family member E, Endothelial leukocyte adhesion molecule 1, ELAM-1, Leukocyte-endothelial cell adhesion molecule 2, LECAM2, CD62E

Gene ID: 6401

UniProt: P16581

Pathways: Thromboxane A2 Receptor Signaling