Selectin E/CD62e antibody (Biotin)

Catalog No. ABIN7836504

This Mouse Monoclonal antibody specifically detects Selectin E/CD62e in FACS and IF. It exhibits reactivity toward Human.

Quick Overview for Selectin E/CD62e antibody (Biotin) (ABIN7836504)

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Selectin E/CD62e antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 1-2B6

Product Details anti-Selectin E/CD62e Antibody (Biotin)

Purpose: Mouse monoclonal antibody Biotin labled to CD62E/CD62P

Specificity: Recognizes human CD62E/CD62P

Purification: The antibody was purified by affinity chromatography.

Immunogen: TNF stimulated human endothelial cells

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled Streptavidin and incubate for 20 minutes away from light at room temperature. 7. Repeat step 5. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for Selectin E/CD62e

Target: Selectin E/CD62e (SELE) (Selectin E (SELE))

Alternative Name: SELE

Background: ELAM1, E-selectin, CD62 antigen-like family member E, Endothelial leukocyte adhesion molecule 1, ELAM-1, Leukocyte-endothelial cell adhesion molecule 2, LECAM2, CD62E, GMRP, GRMP, P-selectin, CD62 antigen-like family member P, Granule membrane protein 140, GMP-140, Leukocyte-endothelial cell adhesion molecule 3, LECAM3, Platelet activation dependent granule-external membrane protein, PADGEM, CD62P

Gene ID: 6401

UniProt: P16581

Pathways: Thromboxane A2 Receptor Signaling