CD69 antibody (PE-Cy5)

Catalog No. ABIN7836553

The Armenian Hamster Monoclonal anti-CD69 antibody is suitable to detect CD69 in samples from Mouse. It has been validated for FACS and IF.

Quick Overview for CD69 antibody (PE-Cy5) (ABIN7836553)

Target: CD69

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This CD69 antibody is conjugated to PE-Cy5

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: H1-2F3

Product Details anti-CD69 Antibody (PE-Cy5)

Purpose: Armenian Hamster monoclonal antibody PE/Cy5 labled to CD69

Specificity: Recognizes mouse CD69

Purification: The antibody was purified by affinity chromatography.

Immunogen: Mouse dendritic epidermal T cell line Y245

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for CD69

Target: CD69

Alternative Name: CD69

Background: CLEC2C, Early activation antigen CD69, Activation inducer molecule, AIM, BL-AC/P26, C-type lectin domain family 2 member C, EA1, Early T-cell activation antigen p60, GP32/28, Leukocyte surface antigen Leu-23, MLR-3, CD69

Gene ID: 12515