CD83 antibody (PE)

Catalog No. ABIN7836632

The PE-conjugated Mouse Monoclonal anti-CD83 antibody (Clone HB15e) (ABIN7836632) specifically detects CD83 in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for CD83 antibody (PE) (ABIN7836632)

Target: CD83

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD83 antibody is conjugated to PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: HB15e

Product Details anti-CD83 Antibody (PE)

Purpose: Mouse monoclonal antibody PE labled to CD83

Specificity: Recognizes human CD83

Purification: The antibody was purified by affinity chromatography.

Immunogen: Native purified human CD83.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for CD83

Target: CD83

Alternative Name: CD83

Background: CD83 antigen, hCD83, B-cell activation protein, Cell surface protein HB15, CD antigen CD83

Gene ID: 9308

UniProt: Q01151