CXCL9 antibody

Catalog No. ABIN7837366

The Armenian Hamster Monoclonal anti-CXCL9 antibody is suitable to detect CXCL9 in samples from Mouse. It has been validated for FACS and IF.

Quick Overview for CXCL9 antibody (ABIN7837366)

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Reactivity: Mouse

Host: Armenian Hamster

Clonality: Monoclonal

Conjugate: This CXCL9 antibody is un-conjugated

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: MIG-2F5-5

Product Details anti-CXCL9 Antibody

Purpose: Armenian Hamster monoclonal antibody to CXCL9

Specificity: Recognizes mouse CXCL9

Purification: The antibody was purified by affinity chromatography.

Immunogen: Mouse CXCL9 Recombinant Protein

Application Details

Application Notes: IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 8. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for CXCL9

Target: CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))

Alternative Name: CXCL9

Background: CMK, MIG, SCYB9, C-X-C motif chemokine 9, Gamma-interferon-induced monokine, Monokine induced by interferon-gamma, HuMIG, MIG, Small-inducible cytokine B9

Gene ID: 17329