PKC beta antibody (pThr500)

Catalog No. ABIN741240

This anti-PKC beta antibody is a Rabbit Polyclonal antibody detecting PKC beta in WB, ELISA, FACS, IF (cc), IF (p), IHC (p) and IHC (fro). Suitable for Human and Rat.

Quick Overview for PKC beta antibody (pThr500) (ABIN741240)

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Reactivity: Human, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PKC beta antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-PKC beta Antibody

Binding Specificity: pThr500

Cross-Reactivity: Human, Rat

Predicted Reactivity: Mouse,Cow,Horse,Chicken,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic peptide derived from human PRKCB around the phosphorylation site of Thr500

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for PKC beta

Target: PKC beta (PRKCB) (Protein Kinase C, beta (PRKCB))

Alternative Name: PRKCB

Background:

Synonyms: PKCB, PRKCB1, PRKCB2, PKC-beta, Protein kinase C beta type, PKC-B, PRKCB

Background: Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase has been reported to be involved in many different cellular functions, such as B cell activation, apoptosis induction, endothelial cell proliferation, and intestinal sugar absorption. Studies in mice also suggest that this kinase may also regulate neuronal functions and correlate fear-induced conflict behavior after stress. Alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq, Jul 2008]

Gene ID: 5579

UniProt: P05771

Pathways: WNT Signaling, TCR Signaling, Thyroid Hormone Synthesis, Nuclear Hormone Receptor Binding, Chromatin Binding, Myometrial Relaxation and Contraction, VEGF Signaling, Unfolded Protein Response, BCR Signaling