H3C1 (H3K27me3) antibody

Catalog No. ABIN7839604

The Rabbit Monoclonal anti-H3C1 antibody is suitable to detect H3C1 in samples from Human and Rat. It has been validated for WB, IF, IHC, ChIP and IP.

Quick Overview for H3C1 (H3K27me3) antibody (ABIN7839604)

Target: H3C1

Reactivity: Human, Rat

Host: Rabbit

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF), Immunohistochemistry (IHC), Chromatin Immunoprecipitation (ChIP), Immunoprecipitation (IP)

Product Details

Binding Specificity: H3K27me3

Purpose: Rabbit monoclonal antibody to Histone H3 (TriMethyl-K27)

Specificity: Recognizes endogenous levels of Histone H3 protein only when Tri-methylated at K27.

Purification: The antibody was purified by immunogen affinity chromatography.

Immunogen: KLH-conjugated synthetic Tri-methylated peptide corresponding to residues surrounding K27 of human Histone H3 protein. The exact sequence is proprietary.

Application Details

Application Notes: WB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/10 - 1/50), ChIP (1/10 - 1/50)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in 50 mM Tris-Glycine ( pH 7.4), 0.15M NaCl, 50 % Glycerol, 0.01 % Sodium azide and 0.05 % BSA.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details

Target: H3C1

Background: H3FA, H3FL, H3FC, H3FB, H3FD, H3FI, H3FH, H3FK, H3FF, H3FJ, Histone H3.1, Histone H3/a, Histone H3/b, Histone H3/c, Histone H3/d, Histone H3/f, Histone H3/h, Histone H3/i, Histone H3/j, Histone H3/k, Histone H3/l

Gene ID: 8350, 291159

UniProt: P68431