LY-49I (LY-49I) antibody (PE)

Catalog No. ABIN7840912

The Mouse Monoclonal anti-LY-49I antibody is suitable to detect LY-49I in samples from Mouse. It has been validated for FACS and IF.

Quick Overview for LY-49I (LY-49I) antibody (PE) (ABIN7840912)

Target: LY-49I

Reactivity: Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: PE

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: YLI-90

Product Details

Purpose: Mouse monoclonal antibody PE labled to Ly-49I

Specificity: Recognizes mouse Ly-49I

Purification: The antibody was purified by affinity chromatography.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details

Target: LY-49I

Alternative Name: Ly49I

Background: MHC class I recognition receptor Ly49I, Natural killer cell receptor Ly49C

Gene ID: 16640