HIST1H2AA antibody (APC)

Catalog No. ABIN7841258

The APC-conjugated Rat Monoclonal anti-HIST1H2AA antibody (Clone NIMR-4) (ABIN7841258) specifically detects HIST1H2AA in IF and FACS. The antibody is reactive with Mouse samples.

Quick Overview for HIST1H2AA antibody (APC) (ABIN7841258)

Target: HIST1H2AA (Histone Cluster 1, H2aa (HIST1H2AA))

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This HIST1H2AA antibody is conjugated to APC

Application: Immunofluorescence (IF), Flow Cytometry (FACS)

Clone: NIMR-4

Product Details anti-HIST1H2AA Antibody (APC)

Purpose: Rat monoclonal antibody APC labled to MHC Class II

Specificity: Recognizes mouse MHC Class II

Purification: The antibody was purified by affinity chromatography.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for HIST1H2AA

Target: HIST1H2AA (Histone Cluster 1, H2aa (HIST1H2AA))

Alternative Name: H2-Aa

Background: Ia1, H2Aa, Ia-1, H-2Aa, Aalpha, IAalpha, I-Aalpha

Gene ID: 14960