TRAC antibody (FITC)

Catalog No. ABIN7845263

The FITC-conjugated Mouse Monoclonal anti-TRAC antibody (Clone IP26) (ABIN7845263) specifically detects TRAC in FACS and IF. The antibody is reactive with Human samples.

Quick Overview for TRAC antibody (FITC) (ABIN7845263)

Target: TRAC (T Cell Receptor alpha Constant (TRAC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TRAC antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: IP26

Product Details anti-TRAC Antibody (FITC)

Purpose: Mouse monoclonal antibody FITC labled to TCR alpha/beta

Specificity: Recognizes human TCR alpha/beta

Purification: The antibody was purified by affinity chromatography.

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for TRAC

Target: TRAC (T Cell Receptor alpha Constant (TRAC))

Alternative Name: TRAC

Background: TCRA, T cell receptor alpha chain constant, T cell receptor beta constant 1

Gene ID: 28755

UniProt: P01848