TdT antibody (FITC)

Catalog No. ABIN7845291

This Mouse Monoclonal antibody specifically detects TdT in FACS and IF. It exhibits reactivity toward Human.

Quick Overview for TdT antibody (FITC) (ABIN7845291)

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This TdT antibody is conjugated to FITC

Application: Flow Cytometry (FACS), Immunofluorescence (IF)

Clone: 41A

Product Details anti-TdT Antibody (FITC)

Purpose: Mouse monoclonal antibody FITC labled to TDT

Specificity: Recognizes human TDT

Purification: The antibody was purified by affinity chromatography.

Immunogen: Recombinant full length human TdT

Application Details

Application Notes: Flow: 10 μL / assay

Protocol: 1. Take 100 μL peripheral blood anticoagulated by EDTA and add to the bottom of 5 mL tube. 2. Add 10 μL labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light. 3. Add 2 mL RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant. 5. Add 2 mL PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant. 6. Add 0.5 mL PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured).

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.3, 0.2 % BSA, and 0.02 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Shipped and store at 4°C for one year. Do not freeze.

Target Details for TdT

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Alternative Name: DNTT

Background: TDT, DNA nucleotidylexotransferase, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal transferase

Gene ID: 1791

UniProt: P04053

Pathways: DNA Damage Repair