Recombinant PKC alpha antibody (pThr638)

Catalog No. ABIN7847695

The Rabbit Monoclonal anti-PKC alpha antibody is suitable to detect PKC alpha in samples from Human and Mouse. It has been validated for WB.

Quick Overview for Recombinant PKC alpha antibody (pThr638) (ABIN7847695)

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Antibody Type: Recombinant Antibody

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PKC alpha antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-PKC alpha Antibody

Binding Specificity: pThr638

Expression System: HEK-293 Cells

Purpose: Recombinant rabbit monoclonal antibody to PKC alpha (Phospho-T638)

Specificity: Recognizes endogenous levels of PKC alpha protein only when phosphorylated at T638

Purification: The antibody was purified by immunogen affinity chromatography.

Immunogen: KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T638 of human PKC alpha protein. The exact sequence is proprietary.

Application Details

Application Notes: WB (1/500 - 1/1000)

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Liquid in PBS, pH 7.4, containing 50 % glycerol, 0.2 % BSA and 0.01 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles.

Target Details for PKC alpha

Target: PKC alpha (PKCa) (Protein Kinase C, alpha (PKCa))

Alternative Name: PRKCA

Background: PKCA, PRKACA, Protein kinase C alpha type, PKC-A, PKC-alpha

Gene ID: 5578, 18750

UniProt: P17252

Pathways: WNT Signaling, TCR Signaling, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Thyroid Hormone Synthesis, cAMP Metabolic Process, Myometrial Relaxation and Contraction, Cell-Cell Junction Organization, Regulation of G-Protein Coupled Receptor Protein Signaling, G-protein mediated Events, Signaling Events mediated by VEGFR1 and VEGFR2, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, VEGFR1 Specific Signals, VEGF Signaling