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Histone 3 antibody (pSer28)

Name: Histone 3 antibody (pSer28)
SKU: ABIN742568
Availability: InStock

The Rabbit Polyclonal anti-Histone 3 antibody is suitable to detect Histone 3 in samples from Human and Rat. It has been validated for WB, ELISA, FACS and IHC (p).

Formalin-fixed and paraffin embedded rat brain labeled with Rabbit Anti Phospho-Histone H3 (Ser28) Polyclonal Antibody, Unconjugated (ABIN742568) at 1:200 followed by conjugation to the secondary antibody and DAB staining (Histone 3 antibody (pSer28))

IHC

Hela cells were fixed with 70% ice-cold methanol overnight at 4℃, permeabilized with 90% ice-cold methanol for 20 min at -20℃, and incubated in 5% BSA blocking buffer for 30 min at room temperature. (Histone 3 antibody (pSer28))

2 images

Catalog No. ABIN742568

$384.62

Plus shipping costs $50.00

100 μL

Shipping to: United States

Delivery in 4 to 7 Business Days

Quick Overview for Histone 3 antibody (pSer28) (ABIN742568)

Target

See all Histone 3 (H3) Antibodies

Histone 3 (H3) (Histone H3 (H3))

Reactivity

1698
1138
1007
48
40
37
35
32
25
23
21
20
11
8
6
6
5
5
4
4
3
3
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1
1

Human, Rat

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Histone 3 antibody is un-conjugated

Application

1356
577
498
413
335
308
205
203
202
188
177
114
113
49
48
37
26
25
19
15
14
7
7
7
4
3
2
1
1
1
1

Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Discover our top product H3 Primary Antibody

Binding Specificity
70

52

51

49

47

46

46

45

44

40

38

38

34

32

32

31

30

29

27

26

26

25

24

24

24

23

22

22

19

19

18

18

16

16

15

15

14

14

13

12

11

11

11

11

11

10

10

10

10

10
pSer28

Specificity

This phosphorylation site is homologous across the listed species.

Cross-Reactivity

Human, Rat

Predicted Reactivity

Mouse,Cow,Pig,Rabbit,Drosophila

Purification

Purified by Protein A.

Immunogen

KLH conjugated synthetic phosphopeptide derived from human Histone H3 around the phosphorylation site of Ser29

Isotype

IgG
anti-Histone H3 (H3) (3meLys4) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq, CUT&RUN Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27ac) antibody
H3 Reactivity: Human, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K9ac) antibody
H3 Reactivity: Human, Mouse WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me3) antibody
H3 Reactivity: Human, Mouse WB, ChIP, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Mouse Monoclonal MABI 0333 unconjugated

anti-Histone H3 (H3) (AA 71-136) antibody
H3 Reactivity: Human, Mouse, Rat WB, ELISA, ICC, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K27me) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K4me3) antibody
H3 Reactivity: Human, Mouse, Saccharomyces cerevisiae WB, IF, ChIP, ICC, DB, ChIP-seq, CUT&RUN, CUT&Tag Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3R2me2) antibody
H3 Reactivity: Human WB, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

anti-Histone H3 (H3) (H3K36me2) antibody
H3 Reactivity: Human WB, IHC, IF, ChIP, IP, ChIP-seq Host: Rabbit Polyclonal unconjugated

Application Notes

WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400

Restrictions

For Research Use only
Format

Liquid

Concentration

1 μg/μL

Buffer

0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative

ProClin

Precaution of Use

This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage

4 °C,-20 °C

Storage Comment

Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date

12 months
Target

Histone 3 (H3) (Histone H3 (H3))

Alternative Name

Histone H3

Background

Synonyms: H3t, H3.4, H3/g, H3FT, Histone H3.1t, H3/t, HIST3H3
Background: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.

Gene ID

8290

UniProt

Q16695