Recombinant PVRL4 antibody (Extracellular Domain)

Catalog No. ABIN7865529

The Rabbit Monoclonal anti-PVRL4 antibody is suitable to detect PVRL4 in samples from Human. It has been validated for IHC and FACS.

Quick Overview for Recombinant PVRL4 antibody (Extracellular Domain) (ABIN7865529)

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This PVRL4 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: NECTIN4-13438R

Product Details anti-PVRL4 Antibody

Binding Specificity: AA 266-280, Extracellular Domain

Expression System: CHO Cells

Purpose: Recombinant Nectin 4 / Nectin Cell Adhesion Molecule 4 / PVRL4 Antibody

Specificity: Homologous to the poliovirus receptor (PVR/CD155), the nectin immunoglobulin superfamily comprises four known isoforms (-1, -2, -3, and -4). The ectodomain of nectin family members comprises three Ig-like domains (V, C, C). Nectins localize at the adherens junctions (AJ) in epithelial and endothelial cells where they serve as adhesion molecules. Actin-based AJs play a role in mechanical adhesion, cellular morphogenesis and cellular differentiation. Nectin associates with the Actin cytoskeleton through its interaction with the Actin filament-binding protein afadin. Nectin 4 and afadin co-localize at cadherin-based adherens junctions in MDCKII epithelial cells. Nectin 4 and Nectin 3 share a common binding region in the V domain of Nectin 1 and thus compete for Nectin 1 binding. The Nectin 3/4 binding domain maps to the C-C'-C''-D b strands of the V domain of Nectin 1. Unlike other nectins, which are more widely expressed, Nectin 4 is mainly expressed in the placenta.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Recombinant fragment (around aa266-280) of human NECTIN4 protein corresponding to the extracellular domain (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PVRL4

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Alternative Name: Nectin 4

Background:

Ig superfamily receptor LNIR family, Nectin 4, poliovirus receptor-related 4, Processed poliovirus receptor-related protein 4, PRR4, PVRL4

Human tonsil, placenta or breast. MCF-7 or T47D cells.

Molecular Weight: 66kDa

Gene ID: 81607, 492490

UniProt: Q96NY8

Pathways: Cell-Cell Junction Organization