Recombinant APC antibody (C-Term)

Catalog No. ABIN7865339

The Mouse Monoclonal anti-APC antibody (Clone rEMM43) (ABIN7865339) specifically detects APC in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant APC antibody (C-Term) (ABIN7865339)

Target: APC (Adenomatous Polyposis Coli (APC))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This APC antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: REMM43

Product Details anti-APC Antibody

Binding Specificity: C-Term

Expression System: CHO Cells

Purpose: Recombinant APC / Adenomatous Polyposis Coli / FAP (Tumor Suppressor) Antibody

Specificity: The human adenomatous polyposis coli (APC) gene at locus 5q21 encodes a protein of 2,843 amino acids. The precise function of this 300 kD protein is not clear but it may have several functions including a role in cell proliferation, apoptosis, expression of cell adhesion proteins and modulation of cell to cell interactions. It is reported to be expressed in the cytoplasm of cell types of many lineages, both epithelial and mesenchymal. In the epithelium of bladder, small and large intestine, esophagus, stomach and epidermis, APC expression is restricted to regions in which cell replication has ceased and terminal differentiation has been established. Expression has been observed in lung, liver, kidney and mammary gland endothelial, myoepithelial and duct lining epithelial cells. Some tissues such as ovary, myometrium, thyroid, parathyroid and tonsil do not express the protein.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Prokaryotic recombinant protein corresponding to a 251 amino acid region of the C-terminus of the human adenomatous polyposis coli protein

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for APC

Target: APC (Adenomatous Polyposis Coli (APC))

Alternative Name: APC

Background:

Adenomatous Polyposis Coli, CC1, Deleted in polyposis 2.5 (DP2.5), DP2, DP3, FAP, FPC, GS, Protein APC

Human tonsil, placenta, breast or colon. HCT116 or COLO 320DM cells.

Molecular Weight: 66kDa

Gene ID: 324, 158932

UniProt: P25054

Pathways: WNT Signaling, Stem Cell Maintenance, Cell-Cell Junction Organization