Recombinant CPS1 antibody

Catalog No. ABIN7857741

The Rabbit Monoclonal anti-CPS1 antibody is suitable to detect CPS1 in samples from Human. It has been validated for WB and IHC.

Quick Overview for Recombinant CPS1 antibody (ABIN7857741)

Target: CPS1 (Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CPS1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: CPS1-9151R

Product Details anti-CPS1 Antibody

Expression System: CHO Cells

Purpose: Recombinant CPS1 / Carbamoyl-Phosphate Synthetase (Hepatocellular Marker) Antibody

Specificity: This MAb recognizes a protein of 165 kDa, identified as carbamoyl phosphate synthetase 1 (CPS1). This mitochondrial enzyme catalyzes synthesis of carbamoyl phosphate from ammonia and bicarbonate. This reaction is the first committed step of the urea cycle, which is important in the removal of excess urea from cells.Deficiency of CPS1 is an autosomal recessive disorder that causes hyperammonemia. CPS1 is a hepatocyte specific protein that localizes to the mitochondria of hepatocytes. It is a sensitive marker for distinguishing hepatocellular carcinomas (HCC) from other metastatic carcinomas as well as cholangio-carcinomas. HCC s occur primarily in the stomach, but they are also found in many other organs. CPS1 may also be a useful marker for intestinal metaplasia. Reportedly, strong expression of CPS1 correlates with smaller tumor size and longer patient survival. Occasionally, CPS1 is also found in gastric carcinomas as well as in a few other non-hepatic tumors.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Recombinant full-length human CPS1 protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for CPS1

Target: CPS1 (Carbamoyl-Phosphate Synthase 1, Mitochondrial (CPS1))

Alternative Name: CPS1

Background:

Carbamoyl-phosphate synthetase 1 (CPS1), Carbamoylphosphate synthetase 1, CPSase 1, CPSASE1

HeLa cells. Liver or Hepatocellular Carcinoma (HCC).

Molecular Weight: ~165kDa

Gene ID: 1373, 149252

UniProt: P31327

Pathways: Response to Growth Hormone Stimulus, Cellular Glucan Metabolic Process