Recombinant CD39 antibody

Catalog No. ABIN7857782

This Mouse Monoclonal antibody specifically detects CD39 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD39 antibody (ABIN7857782)

Target: CD39 (ENTPD1) (Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD39 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: R22A9

Product Details anti-CD39 Antibody

Expression System: CHO Cells

Purpose: Recombinant CD39 / ENTPD1 (Marker of Tumor-specific T cells) Antibody

Specificity: CD39 is a transmembrane glycoprotein and an extracellular nucleotide-hydrolyzing enzyme that plays a key role in regulating extracellular ATP and ADP levels. Also known as E-type apyrase, CD39 hydrolyzes ATP and ADP to AMP, which is further converted to adenosine. By degrading ADP, CD39 inhibits platelet aggregation, contributing to its anti-thrombotic function and potential use in preventing coronary artery occlusion and thrombotic stroke. Intracellularly, CD39 undergoes glycosylation at six N-glycosylation sites before translocating to the membrane to become an active enzyme. Alternative splicing gives rise to three isoforms: vascular, placenta I, and placenta II, with variations at the N-terminus and C-terminus. CD39 is expressed in vascular tissues, including the placenta, lung, skeletal muscle, and kidney, as well as on endothelial cells, smooth muscle cells, cardiac cells, and platelets. In the immune system, CD39 is present on activated B cells, a subset of activated T cells, activated NK cells, macrophages, dendritic cells, and Langerhans cells. It is not expressed on resting lymphocytes but is found in lymphoid tissues, particularly in the mantle zone and paracortical lymphocytes. CD39-expressing cells may provide protection to lymphocytes from the toxic effects of ATP released from damaged cells. Originally identified on Epstein-Barr virus-transformed B cells, CD39 is now recognized as a key immunoregulatory enzyme involved in inflammation, immune suppression, and thrombotic balance.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Prokaryotic recombinant protein corresponding to a portion of the external domain of the CD39 molecule

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for CD39

Target: CD39 (ENTPD1) (Ectonucleoside Triphosphate diphosphohydrolase 1 (ENTPD1))

Alternative Name: CD39

Background:

ATPDase, DKFZp686D194, DKFZp686I093, Ecto apyrase, Ecto ATP diphosphohydrolase, Ectonucleoside triphosphate diphosphohydrolase 1, ENTPD1, FLJ40921, FLJ40959, Lymphoid cell activation antigen, NTPDase 1, SPG64

Human tonsil, placenta, kidney or lymph node.

Molecular Weight: 70-100 kDa

Gene ID: 953, 576612

UniProt: P49961