Recombinant Involucrin antibody

Catalog No. ABIN7864812

The Mouse Monoclonal anti-Involucrin antibody (Clone rIVRN-9323) (ABIN7864812) specifically detects Involucrin in IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant Involucrin antibody (ABIN7864812)

Target: Involucrin (IVL)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Involucrin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: RIVRN-9323

Product Details anti-Involucrin Antibody

Expression System: CHO Cells

Purpose: Recombinant Involucrin (Squamous Cell Terminal Differentiation Marker) Antibody

Specificity: It recognizes a protein of 66 kDa-170 kDa, identified as involucrin. In Western blotting of cultured human keratinocytes, this MAb reacts with a 120 kDa protein. It stains the involucrin in a variety of sizes: 170 kDa in MCF-7 cells, a doublet of ~115 kDa and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of 105 kDa in pig. Its epitope maps between codon 421-568 of human involucrin. Involucrin is expressed in a range of stratified squamous epithelia, including the cornea, which lacks a distinct cornified layer. In normal epidermis, it is first expressed in the upper spinous layers, and in keratinocyte cultures, all cells that have left the basal layer express it. Involucrin expression is altered in pathological conditions: in psoriasis and other benign epidermal hyperplasias, involucrin expression begins closer to the basal layer than normal, expression is abnormal in squamous cell carcinomas and premalignant lesions, and is reduced in severe dysplasias of the larynx and cervix.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Recombinant human full-length Involucrin protein (IVRN)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Involucrin

Target: Involucrin (IVL)

Alternative Name: Involucrin

Background:

IVL

MCF-7 cells. Localized to upper spinous and granular layers in normal skin.

Molecular Weight: 66-170kDa

Gene ID: 3713, 516439

UniProt: P07476