Laminin gamma 1 antibody

Catalog No. ABIN7865547

The Rabbit Monoclonal anti-Laminin gamma 1 antibody (Clone LAMC1-13970R) (ABIN7865547) specifically detects Laminin gamma 1 in IHC. The antibody is reactive with Human samples.

Quick Overview for Laminin gamma 1 antibody (ABIN7865547)

Target: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Laminin gamma 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: LAMC1-13970R

Product Details anti-Laminin gamma 1 Antibody

Expression System: CHO Cells

Purpose: Laminin, gamma 1 (LAMC1) Antibody

Specificity: Laminins are large hetero-trimeric, non-collagenous glycoproteins composed of alpha, beta, and gamma chains. This MAb reacts with laminin B2/1 chain of ~210 kDa and does not cross-react with other basement membrane components or fibronectin. Its specificity was established by immunoprecipitation and immunofluorescence of human skeletal muscle and kidney with laminin chain-specific MAbs. Epithelial sheets in vivo are separated from the mesenchymal elements of the stroma by a thin layer of a specialized type of extracellular matrix termed the basement membrane (BM). This structure consists of individual components, some of which are ubiquitous in BMs and some are not. The ubiquitous ones comprise laminin (LN), entactin/nidogen (EN), collagen type IV (CIV), and large heparan sulfate proteoglycan (HSPG), which interact specifically with each other to form a continuous and regular BM. Alterations of BM integrity, from local discontinuities up to complete loss, are described in many types of human and animal epithelial neoplasms. This MAb stains uniformly all human and murine basement membranes.

Purification: 200ug/ml of Ab produced in CHO cell mammalian-based expression system.

Immunogen: Recombinant human full-length LAMC1 protein

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Laminin gamma 1

Target: Laminin gamma 1 (LAMC1) (Laminin, gamma 1 (LAMC1))

Alternative Name: Laminin, gamma 1

Background:

LAMB2, LAMC1, Laminin B2 chain, Laminin gamma 1, Laminin gamma 1 chain

Normal colon or carcinomas.

Molecular Weight: 210kDa

Gene ID: 3915

UniProt: P11047