Recombinant Caldesmon HMW antibody

Catalog No. ABIN7857868

The Mouse Monoclonal anti-Caldesmon HMW antibody (Clone rCALD1-7266) (ABIN7857868) specifically detects Caldesmon HMW in IHC and WB. The antibody is reactive with Human samples.

Quick Overview for Recombinant Caldesmon HMW antibody (ABIN7857868)

Target: Caldesmon HMW

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: Un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: RCALD1-7266

Product Details

Expression System: HEK-293 Cells

Purpose: Recombinant Caldesmon, HMW (h-Caldesmon) (Smooth Muscle Marker) Antibody

Specificity: Recognizes a protein of 150 kDa, which is identified as the high molecular weight variant of Caldesmon. Two closely related variants of human caldesmon have been identified which are different in their electrophoretic mobility and cellular distribution. The h-caldesmon variant (120 150 kDa) is predominantly expressed in smooth muscle whereas l-caldesmon (70 80 kDa) is found in non- muscle tissue and cells. Neither of the two variants has been detected in skeletal muscle. This MAb recognizes only the 150 kDa variant (h-caldesmon) in Western blots of human aortic media extracts and is unreactive with fibroblast extracts from cultivated human foreskin. Caldesmon is a developmentally regulated protein involved in smooth muscle and non-muscle contraction.

Purification: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system.

Immunogen: Recombinant human full-length CALD1 protein

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details

Target: Caldesmon HMW

Alternative Name: Caldesmon, HMW

Background:

CAD, CALD1, Caldesmon 1 Isoform 1, Caldesmon 1 Isoform 2, Caldesmon 1 Isoform 3, Caldesmon 1 Isoform 4, Caldesmon 1 Isoform 5, CDM, HCAD, LCAD, NAG22

Blood vessels in all tissues, smooth muscle or leiomyosarcoma.

Molecular Weight: 150kDa

Gene ID: 800, 490203

UniProt: Q05682