Recombinant HBA1 antibody (AA 1-100)

Catalog No. ABIN7864747

The Mouse Monoclonal anti-HBA1 antibody is suitable to detect HBA1 in samples from Human. It has been validated for IHC.

Quick Overview for Recombinant HBA1 antibody (AA 1-100) (ABIN7864747)

Target: HBA1 (Hemoglobin, alpha 1 (HBA1))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This HBA1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: RHBA-9167

Product Details anti-HBA1 Antibody

Binding Specificity: AA 1-100

Purpose: Recombinant Hemoglobin alpha (2 chain) Antibody

Specificity: Hemoglobin (Hgb) is coupled to four iron-binding, methene-linked tetrapyrrole rings (heme). The ± (16p13.3; 5'-é 79oΩ-pseudoé 79oΩ-pseudo±2-pseudo±1-±2-±1- é 79oΩ1-3') and ≤ (11p15.5) globin loci determine the basic Hgb structure. The globin portion of Hgb consists of two ± chains and two ≤ chains arranged in pairs forming a tetramer. Each of the four globin chains covalently associates with a heme group. The bonds between ± and ≤ chains are weaker than between similar globin chains, thereby forming a cleavage plane that is important for oxygen binding and release. High affinity for oxygen occurs upon relaxation of the ±1-≤2 cleavage plane. When the two ±1-≤2 interfaces are closely bound, Hgb has a low affinity for oxygen. Hb A, which contains two ± chains plus two ≤ chains, comprises 97% of total circulating hemoglobin. The remaining 3% of total circulating hemoglobin is comprised of Hb A-2, which consists of two ± chains plus two d chains, and fetal hemoglobin (Hb F), which consists of two ± chains together with two g chains.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-100) of human HBA2 protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for HBA1

Target: HBA1 (Hemoglobin, alpha 1 (HBA1))

Alternative Name: Hemoglobin alpha

Background:

Alpha 1 globin, HBA1, HBA2, Hemoglobin alpha 1 globin chain, Hemoglobin alpha 2, Hemoglobin subunit alpha

Human spleen, stomach cancer, lung or placenta.

Molecular Weight: 16kDa

Gene ID: 3039, 449630

UniProt: P69905