IRAK1 antibody (pSer376)

Catalog No. ABIN742643

The Rabbit Polyclonal anti-IRAK1 antibody is suitable to detect IRAK1 in samples from Human and Mouse. It has been validated for WB, ELISA, FACS, IF (cc), IF (p), IHC (p) and IHC (fro).

Quick Overview for IRAK1 antibody (pSer376) (ABIN742643)

Target: IRAK1 (Interleukin-1 Receptor-Associated Kinase 1 (IRAK1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IRAK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Flow Cytometry (FACS), Immunofluorescence (Cultured Cells) (IF (cc)), Immunofluorescence (Paraffin-embedded Sections) (IF (p)), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Product Details anti-IRAK1 Antibody

Binding Specificity: pSer376

Cross-Reactivity: Human, Mouse

Predicted Reactivity: Rat,Dog,Rabbit

Purification: Purified by Protein A.

Immunogen: KLH conjugated synthetic phosphopeptide derived from mouse IRAK1 around the phosphorylation site of Ser376 [QS(p-S)TV]

Isotype: IgG

Application Details

Application Notes: WB 1:300-5000
ELISA 1:500-1000
FCM 1:20-100
IHC-P 1:200-400
IHC-F 1:100-500
IF(IHC-P) 1:50-200
IF(IHC-F) 1:50-200
IF(ICC) 1:50-200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 μg/μL

Buffer: 0.01M TBS( pH 7.4) with 1 % BSA, 0.02 % Proclin300 and 50 % Glycerol.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE, which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Shipped at 4°C. Store at -20°C for one year. Avoid repeated freeze/thaw cycles.

Expiry Date: 12 months

Target Details for IRAK1

Target: IRAK1 (Interleukin-1 Receptor-Associated Kinase 1 (IRAK1))

Alternative Name: IRAK1

Background:

Synonyms: IRAK1 Ser376, IRAK1 S376, IRAK1 p-Ser376, Il1rak, Il1rak, Interleukin 1 receptor associated kinase 1, Interleukin 1 receptor associated kinase 1, Interleukin 1 receptor associated kinase 2, Interleukin-1 receptor-associated kinase 1, IRAK, IRAK-1, IRAK1, IRAK1, IRAK1_HUMAN, IRAK2, IRAK2, mPLK, mPLK, Pelle, Pelle, Pelle homolog, Pelle-like protein kinase, Plpk.

Background: IRAK or Interleukin-1 Receptor-associated Kinase 1, is one of two putative serine/threonine kinases that become associated with the interleukin-1 receptor (IL1R) upon stimulation. This protein is partially responsible for IL1-induced upregulation of the ubiquitous transcription factor NF-kappa B.

Gene ID: 16179

Pathways: NF-kappaB Signaling, TLR Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Toll-Like Receptors Cascades