Recombinant Ubiquitin antibody (AA 1-119)

Catalog No. ABIN7858186

The Mouse Monoclonal anti-Ubiquitin antibody (Clone rUBB-9476) (ABIN7858186) specifically detects Ubiquitin in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant Ubiquitin antibody (AA 1-119) (ABIN7858186)

Target: Ubiquitin (Ub)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Ubiquitin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: RUBB-9476

Product Details anti-Ubiquitin Antibody

Binding Specificity: AA 1-119

Purpose: Recombinant Ubiquitin (Autophagy Marker) Antibody

Specificity: Ubiquitin is a highly conserved and plays an essential role in the ubiquitin-proteasome pathway. In ubiquitination process, it is first activated by forming a thiol-ester complex with the activation component E1, which is then transferred to ubiquitin-carrier protein E2, followed by to ubiquitin ligase E3 for final delivery to epsilon-NH2 of the target protein lysine residue. IkB, p53, cdc25A, Bcl-2 etc. are shown as targets of ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response, and apoptosis. Moreover, ubiquitin have been reported to bind covalently with pathological inclusions which are resistant to degradation e.g. neurofibrillary tangles/paired helical filaments in Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease etc.

Purification: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment of human Ubiquitin protein (around aa 1-119) (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Ubiquitin

Target: Ubiquitin (Ub)

Alternative Name: Ubiquitin

Background:

Epididymis secretory protein Li 50, Polyubiquitin B, RPS27A, UBA52, UBA80, UBB, UBC, UBCEP1, UBCEP2, Ubiquitin, Ubiquitin B

HeLa or Raji cells, Human Brain.

Molecular Weight: 9kDa

Gene ID: 7314, 356190, 520348

UniProt: P62979, P62987, P62988

Pathways: Mitotic G1-G1/S Phases, Ubiquitin Proteasome Pathway