CD3D antibody (AA 1-171)

Catalog No. ABIN7863949

The Mouse Monoclonal anti-CD3D antibody is suitable to detect CD3D in samples from Human. It has been validated for WB and IHC.

Quick Overview for CD3D antibody (AA 1-171) (ABIN7863949)

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3D antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: CD3D-8898

Product Details anti-CD3D Antibody

Binding Specificity: AA 1-171

Purpose: CD3D / T Cell Receptor delta Antibody

Specificity: The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of g and e chains (ge), a heterodimer of d and e chains (de) and a homodimer of two z chains (zz) or a heterodimer of z and h chains (zh). The z and h chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The g, e and d chains each contain a single copy of a conserved immunoreceptor tyrosine-based activation motif (ITAM). In contrast, the z chain contains three consecutive copies of the same motif. Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk and are also capable of regulating their kinase activity. The crystal structure of the ZAP-70 SH2 domains bound to the z chain ITAMs has been solved.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-171) of human CD3D protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for CD3D

Target: CD3D (CD3d Molecule, delta (CD3-TCR Complex) (CD3D))

Alternative Name: CD3D

Background:

CD3 antigen delta subunit, CD3 delta, CD3d antigen delta polypeptide (TiT3 complex), CD3d molecule delta (CD3-TCR complex), IMD19, OKT3 delta chain, T-cell receptor T3 delta chain, T-cell surface glycoprotein CD3 delta chain, T3D

Human peripheral blood. Jurkat cells. Tonsil or lymph node. MOLT-4.

Molecular Weight: 20kDa

Gene ID: 915, 504048

UniProt: P04234

Pathways: TCR Signaling, CXCR4-mediated Signaling Events