Centrin 1 antibody (AA 1-172)

Catalog No. ABIN7865279

This Mouse Monoclonal antibody specifically detects Centrin 1 in WB and IHC. It exhibits reactivity toward Human.

Quick Overview for Centrin 1 antibody (AA 1-172) (ABIN7865279)

Target: Centrin 1 (CETN1) (Centrin, EF-Hand Protein, 1 (CETN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Centrin 1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: CETN1-9877

Product Details anti-Centrin 1 Antibody

Binding Specificity: AA 1-172

Purpose: CETN1 / Centrin-1 Antibody

Specificity: Centrin-1 Antibody (2A6) is an IgG₁X mouse monoclonal Centrin-1 antibody (also designated Caltractin isoform 2 antibody or CETN1 antibody) that detects the Centrin-1 protein of human origin by WB, IP and ELISA. Centrin-1 Antibody (2A6) is available as the non-conjugated anti-Centrin-1 antibody. EF-hand type Ca2+-binding proteins consists of several family members, including Centrin-1, Centrin-2 and Centrin-3. The Centrin proteins are ubiquitously expressed cytoskeletal components that show increased expression during cell differentiation. Tissues where cilia are present, such as the retina and testis, express both Centrin-1 and -2, but Centrin-2 is also expressed in nondifferentiated, nonciliated retinal cells (retinoblastoma cells), liver, skeletal muscle, and cardiac muscle. In these tissues, Centrin associates with the centrosomes, mitotic spindle poles, and basal bodies. Knockdown studies reveal a requirement for Centrin in centriole duplication and organization of spindle pole morphology and the completion of cytokinesis. Centrin-3 plays a role in centrosome reproduction.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-172) of human CETN1 protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for Centrin 1

Target: Centrin 1 (CETN1) (Centrin, EF-Hand Protein, 1 (CETN1))

Alternative Name: CETN1

Background:

CEN1, CETN

Human testis.

Molecular Weight: 20KDa

Gene ID: 1068, 122511

UniProt: Q12798