CD3G antibody (AA 1-182)

Catalog No. ABIN7865059

The Mouse Monoclonal anti-CD3G antibody (Clone CD3G-8794) (ABIN7865059) specifically detects CD3G in IHC. The antibody is reactive with Human samples.

Quick Overview for CD3G antibody (AA 1-182) (ABIN7865059)

Target: CD3G (CD3g Molecule, gamma (CD3-TCR Complex) (CD3G))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD3G antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: CD3G-8794

Product Details anti-CD3G Antibody

Binding Specificity: AA 1-182

Purpose: CD3G / CD3beta1 / TCR beta Antibody

Specificity: The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multisubunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers: a heterodimer of g and e chains (ge), a heterodimer of d and e chains (de) and a homodimer of two z chains (zz) or a heterodimer of z and h chains (zh). The z and h chains are encoded by the same gene but differ in their carboxyl-terminal ends due to an alternative splicing event. The g, e and d chains each contain a single copy of a conserved immunoreceptor tyrosinebased activation motif (ITAM). In contrast, the z chain contains three consecutive copies of the same motif. Phosphorylated ITAMs act as docking sites for protein kinases such as ZAP-70 and Syk and are also capable of regulating their kinase activity. The crystal structure of the ZAP-70 SH2 domains bound to the z chain ITAMs has been solved.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-182) of human CD3G protein (exact sequence is proprietary)

Isotype: IgG2c, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for CD3G

Target: CD3G (CD3g Molecule, gamma (CD3-TCR Complex) (CD3G))

Alternative Name: CD3G

Background:

CD3g antigen gamma polypeptide (TiT3 complex), CD3g molecule gamma (CD3 TCR complex), T cell antigen receptor complex gamma subunit of T3, T-cell surface glycoprotein CD3 gamma chain, T3G

Human colon|tonsil or spleen.

Molecular Weight: 18-28kDa

Gene ID: 917, 2259

UniProt: P09693

Pathways: TCR Signaling, CXCR4-mediated Signaling Events