Recombinant CD163 antibody (AA 1-200)

Catalog No. ABIN7858386

The Mouse Monoclonal anti-CD163 antibody is suitable to detect CD163 in samples from Human. It has been validated for IHC.

Quick Overview for Recombinant CD163 antibody (AA 1-200) (ABIN7858386)

Target: CD163

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD163 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: RM130-8820

Product Details anti-CD163 Antibody

Binding Specificity: AA 1-200

Purpose: Recombinant CD163 (Monocyte & Macrophage Marker) Antibody

Specificity: CD163 is a type I membrane protein, and is a member of the hemoglobin scavenger receptor cystein-rich superfamily. The protein is involved in the clearance of hemoglobin-haptoglobin complexes and is considered to have anti-inflammatory functions. CD163 expression is restricted to the monocytic/macrophage lineage. It is expressed by all circulating monocytes and by a majority of tissue macrophages, such as splenic dendrocytes, alveolar macrophages and Kupffer cells of the liver. It is not present in macrophages in the mantle zone and some of the germinal center cells in lymph follicles, nor in Langerhans cells and interdigitating reticulum cells. In tumor tissues, CD163 is found in almost all cases of acute myeloid leukemia with monocytoid differentiation and in the majority of cases of histiocytic sarcoma, littoral cell angioma, Rosai-Dorfman disease, Langerhans cell histiocytosis and typical and atypical fibrous histiocytoma. It is also expressed in some cases of dermatofibrosarcoma protuberans. CD163 can be used to detect cells of monocytic and histiocyte lineage in neoplastic and reactive lesions. It has been shown to be more sensitive than CD68 for the detection of macrophages and monocytic cells. It covers a similar, but not identical, spectrum of cells as CD68.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 1-200) of human CD163 protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for CD163

Target: CD163

Alternative Name: CD163

Background:

Hemoglobin scavenger receptor, MM130, Scavenger receptor cysteine rich type 1 protein M130

hPBL cells. Skin|lung|placenta or histiocytoma tissue.

Molecular Weight: 140kDa

Gene ID: 9332, 504641

UniProt: Q86VB7