Recombinant CD3 epsilon antibody (AA 1-200)

Catalog No. ABIN7858396

The Rabbit Monoclonal anti-CD3 epsilon antibody (Clone C3e-8115R) (ABIN7858396) specifically detects CD3 epsilon in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant CD3 epsilon antibody (AA 1-200) (ABIN7858396)

Target: CD3 epsilon (CD3E)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: C3e-8115R

Product Details anti-CD3 epsilon Antibody

Binding Specificity: AA 1-200

Purpose: Recombinant CD3e (T-Cell Marker) Antibody

Specificity: Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28 kDa. The CD3 complex is closely associated at the lymphocyte cell surface with the T cell antigen receptor (TCR). Reportedly, CD3 complex is involved in signal transduction to the T cell interior following antigen recognition. The CD3 antigen is first detectable in early thymocytes and probably represents one of the earliest signs of commitment to the T cell lineage. In cortical thymocytes, CD3 is predominantly intra-cytoplasmic. However, in medullary thymocytes, it appears on the T cell surface. CD3 antigen is a highly specific marker for T cells, and is present in majority of T cell neoplasms.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-200) of human CD3e protein (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Background:

CD3 epsilon, CD3 TCR complex, T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex

Human peripheral blood. Jurkat cells. Tonsil or lymph node. MOLT-4.

Molecular Weight: 20kDa

Gene ID: 916, 3003

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway