PVRL4 antibody (AA 1-200)

Catalog No. ABIN7858662

The Mouse Monoclonal anti-PVRL4 antibody is suitable to detect PVRL4 in samples from Human. It has been validated for IHC.

Quick Overview for PVRL4 antibody (AA 1-200) (ABIN7858662)

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PVRL4 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: NECT4-7271

Product Details anti-PVRL4 Antibody

Binding Specificity: AA 1-200

Purpose: Nectin 4 / Nectin Cell Adhesion Molecule 4 / PVRL4 Antibody

Specificity: Homologous to the poliovirus receptor (PVR/CD155), the nectin immunoglobulin superfamily comprises four known isoforms (-1, -2, -3, and -4). The ectodomain of nectin family members comprises three Ig-like domains (V, C, C). Nectins localize at the adherens junctions (AJ) in epithelial and endothelial cells where they serve as adhesion molecules. Actin-based AJs play a role in mechanical adhesion, cellular morphogenesis and cellular differentiation. Nectin associates with the actin cytoskeleton through its interaction with the actin filament-binding protein afadin. Nectin 4 and afadin co-localize at cadherin-based adherens junctions in MDCKII epithelial cells. Nectin 4 and nectin 3 share a common binding region in the V domain of Nectin 1 and thus compete for Nectin 1 binding. The Nectin 3/4 binding domain maps to the C-C'-C''-D æ strands of the V domain of Nectin 1. Unlike other nectins, which are more widely expressed, Nectin 4 is mainly expressed in the placenta.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-200) of human NECTIN4 protein (exact sequence is proprietary)

Isotype: IgG2a, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PVRL4

Target: PVRL4 (Poliovirus Receptor-Related 4 (PVRL4))

Alternative Name: Nectin 4

Background:

Ig superfamily receptor LNIR family, Nectin 4, poliovirus receptor-related 4, Processed poliovirus receptor-related protein 4, PRR4, PVRL4

Human tonsil, placenta or breast.

Molecular Weight: 66kDa

Gene ID: 81607, 492490

UniProt: Q96NY8

Pathways: Cell-Cell Junction Organization