SPARC antibody (AA 1-200)

Catalog No. ABIN7858698

The Mouse Monoclonal anti-SPARC antibody is suitable to detect SPARC in samples from Human. It has been validated for IHC.

Quick Overview for SPARC antibody (AA 1-200) (ABIN7858698)

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SPARC antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: OSTN-3756

Product Details anti-SPARC Antibody

Binding Specificity: AA 1-200

Purpose: SPARC / Osteonectin Antibody

Specificity: SPARC (for secreted protein acidic and rich in cysteine) is a phosphorylated, acidic, glycine-rich glycoprotein that is secreted by endothelial cells and is present in large amounts in the parietal endoderm of mouse embryos and in human placenta. It is identical to osteonectin, a protein important to bone calcification that is highly conserved between species. SPARC, which can be selectively expressed by the endothelium in response to certain types of injury, induces rounding in adherent endothelial cells in vitro. It regulates endothelial barrier function through F-Actin-dependent changes in cell shape, coincident with the appearance of intercellular gaps, which provide a paracellular pathway for extravasation of macromolecules.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-200) of human SPARC protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide store at 2 to 8°C. Antibody without azide store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for SPARC

Target: SPARC (Secreted Protein, Acidic, Cysteine-Rich (Osteonectin) (SPARC))

Alternative Name: SPARC

Background:

Basement membrane protein 40, BM40, Cysteine rich protein, OI17, ON, Osteonectin, Secreted acidic cystein rich glycoprotein, Secreted protein acidic and cysteine rich, Secreted protein acidic and rich in cysteine (SPARC)

Human placenta|lymph node or spleen. JEG3 or A549 cell lysates.

Molecular Weight: 38kDa

Gene ID: 6678, 111779, 708558

UniProt: P09486

Pathways: Autophagy