T-Bet antibody (AA 1-200)

Catalog No. ABIN7864466

The Mouse Monoclonal anti-T-Bet antibody is suitable to detect T-Bet in samples from Human. It has been validated for IHC.

Quick Overview for T-Bet antibody (AA 1-200) (ABIN7864466)

Target: T-Bet

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This T-Bet antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: TBX21-6724

Product Details anti-T-Bet Antibody

Binding Specificity: AA 1-200

Purpose: T-bet / Tbox-bet / TBX21 Antibody

Specificity: T helper (Th) lymphocytes differentiate into two unique subsets, Th1 and Th2, which differ both in function and in the cytokines they secrete. Th1 and Th2 cytokines promote the growth and differentiation of their subset, and inhibit the growth and differentiation of the opposing subset. T-bet (T-box expressed in T cells) is a Th1-specific T-box transcription factor that controls the expression of the Th1 cytokine, IFN-g. T-bet also converts effector Th2 cells into the opposing Th1 subset. T-bet is selectively expressed in Th1 cells. The level of T-bet expression is increased by signals mediated by the T cell receptor (TCR). IL-12 also induces an increase in the level of T-bet. T-bet was originally isolated from nuclear extracts of resting and PMA/ionomycin-activated AE7 cells. It is expressed in low levels in AE7 cells, and in increased levels in stimulated AE7.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa 1-200) of human TBX21 protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for T-Bet

Target: T-Bet

Background:

T box expressed in T cells, T box protein 21, T box transcription factor TBX21, T cell specific T box transcription factor T bet, T PET, T-cell-specific T-box transcription factor T-bet, TBET, TBLYM, TPET, Transcription factor TBLYM

Human tonsil|non-Hodgkin s lymphoma or spleen. T-cell specific.

Molecular Weight: 62kDa

Gene ID: 30009, 272409

UniProt: Q9UL17