CEA antibody (AA 1-300)

Catalog No. ABIN7858816

The Mouse Monoclonal anti-CEA antibody (Clone CEACAM5-12673) (ABIN7858816) specifically detects CEA in IHC and WB. The antibody is reactive with Human samples.

Quick Overview for CEA antibody (AA 1-300) (ABIN7858816)

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This CEA antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB)

Clone: CEACAM5-12673

Product Details anti-CEA Antibody

Binding Specificity: AA 1-300

Purpose: CEA / CD66e / CEACAM5 (Gastrointestinal Cancer Marker) Antibody

Specificity: This antibody recognizes proteins of 80-200 kDa, identified as different members of CEA family. CEA is synthesized during development in the fetal gut and is re-expressed in increased amounts in intestinal carcinomas and several other tumors. This MAb does not react with nonspecific cross-reacting antigen (NCA) and with human polymorphonuclear leucocytes. It shows no reaction with a variety of normal tissues and is suitable for staining of formalin/paraffin tissues. CEA is not found in benign glands, stroma, or malignant prostatic cells. Antibody to CEA is useful in detecting early foci of gastric carcinoma and in distinguishing pulmonary adenocarcinomas (60-70 % are CEA+) from pleural mesotheliomas (rarely or weakly CEA+). Anti-CEA positivity is seen in adenocarcinomas from the lung, colon, stomach, esophagus, pancreas, gallbadder, urachus, salivary gland, ovary, and endocervix.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1-300) of human CEACAM5 protein (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: Prepared in 10  mM PBS

Preservative: Azide free

Storage: -20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CEA

Target: CEA (Carcinoembryonic Antigen Gene Family (CEA))

Alternative Name: CEA

Background:

Carcinoembryonic Antigen-related Cell Adhesion Molecule 5, CEACAM5, CD66, Biliary Glycoprotein (BGP-1)

MCF7 or 293T cells. Human colon carcinoma or Human Intestine.

Molecular Weight: 80-200kDa

Gene ID: 1048, 709196

UniProt: P06731