Recombinant CD3 epsilon antibody (AA 100-200)

Catalog No. ABIN7863583

The Rabbit Monoclonal anti-CD3 epsilon antibody (Clone C3e-4653R) (ABIN7863583) specifically detects CD3 epsilon in WB and IHC. The antibody is reactive with Human samples.

Quick Overview for Recombinant CD3 epsilon antibody (AA 100-200) (ABIN7863583)

Target: CD3 epsilon (CD3E)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This CD3 epsilon antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: C3e-4653R

Product Details anti-CD3 epsilon Antibody

Binding Specificity: AA 100-200

Purpose: Recombinant CD3e (T-Cell Marker) Antibody

Specificity: Cluster of differentiation 3 (CD3) is a multimeric protein complex, known historically as the T3 complex, and is composed of four distinct polypeptide chains, epsilon ( ), gamma (Á), delta ( 79oΩL) and zeta ( 79oΩÊTO), that assemble and function as three pairs of dimers ( Á, 79oΩL, 79oΩÊTO 79oΩÊTO). The CD3 complex serves as a T cell co-receptor that associates noncovalently with the T cell receptor (TCR).CD3 antigen appearance at all stages of T cell development, and makes it an ideal T cell marker for both the detection of normal T cells and T cell neoplasms (lymphomas and leukemias).

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant human CD3e fragment (around aa100-200) (exact sequence is proprietary)

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for CD3 epsilon

Target: CD3 epsilon (CD3E)

Alternative Name: CD3e

Background:

CD3 epsilon, CD3 TCR complex, T cell antigen receptor complex epsilon subunit of T3, T-cell surface antigen T3/Leu-4 epsilon chain, T-cell surface glycoprotein CD3 epsilon chain, T3E, TCRE, TiT3 complex

Human tonsil, spleen or lymph node. Human peripheral blood or Jurkat cells.

Molecular Weight: 20-25kDa

Gene ID: 916, 3003

UniProt: P07766

Pathways: TCR Signaling, CXCR4-mediated Signaling Events, Ubiquitin Proteasome Pathway