Haptoglobin antibody (AA 100-250)

Catalog No. ABIN7864767

The Mouse Monoclonal anti-Haptoglobin antibody is suitable to detect Haptoglobin in samples from Human. It has been validated for WB and IHC.

Quick Overview for Haptoglobin antibody (AA 100-250) (ABIN7864767)

Target: Haptoglobin (HP)

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Haptoglobin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Clone: HP-4813

Product Details anti-Haptoglobin Antibody

Binding Specificity: AA 100-250

Purpose: Haptoglobin (HP) Antibody

Specificity: Haptoglobin (Hp) is a blood plasma protein that functions to bind free Hemoglobin that has been released from erythrocytes, thereby inhibiting its oxidative activity. During this process, Haptoglobin sequesters the iron within Hemoglobin, preventing iron-utilizing bacteria from benefitting from hemolysis. This function suggests that Haptoglobin concentrations may increase in response to inflammation. The resulting Haptoglobin-Hemoglobin complex is then removed by the reticulo-endothelial system. Due to cleavage of a common precursor protein during protein synthesis, Haptoglobin consists of two a and two b chains, connected by disulfide bridges. In human, Haptoglobin exists in two allelic forms designated Haptoglobin 1 (Hp1) and Haptoglobin 2 (Hp2), where Hp2 is the result of a partial Hp1 gene duplication. There are three known phenotypes of human Haptoglobin: Hp1-1, Hp2-1 and Hp2-2, which may be associated with diabetes and cardiovascular disease pathology and a susceptibility to Parkinson s and Crohn s disease. Haptoglobin levels are useful in diagnosing hemolytic anemia, the abnormal breakdown of red blood cells. Haptoglobin is expressed in mammalian hepatocytes as well as other tissues such as skin, lung and kidney.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa100-250) of human HP protein (exact sequence is proprietary)

Isotype: IgG2, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Haptoglobin

Target: Haptoglobin (HP)

Alternative Name: Haptoglobin

Background:

Binding peptide (BP), Haptoglobin alpha chain / alpha(1S) beta, Haptoglobin alpha(2FS) beta, Haptoglobin beta chain, Haptoglobin, alpha polypeptide, Haptoglobin, beta polypeptide, HP, HP2 ALPHA2, HPA1S, HPT, MGC111141, Zonulin

Human liver.

Molecular Weight: 15-18 kDa ( chain), 45kDa ( chain)

Gene ID: 3240, 513711, 702099

Pathways: Transition Metal Ion Homeostasis