PKC iota antibody (AA 100-300)

Catalog No. ABIN7863285

This Mouse Monoclonal antibody specifically detects PKC iota in IHC. It exhibits reactivity toward Human.

Quick Overview for PKC iota antibody (AA 100-300) (ABIN7863285)

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PKC iota antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: PRKCI-4912

Product Details anti-PKC iota Antibody

Binding Specificity: AA 100-300

Purpose: Protein Kinase C iota / lambda / PRKCI Antibody

Specificity: Members of the protein kinase C (PKC) family play a key regulatory role in a variety of cellular functions, including cell growth and differentiation, gene expression, hormone secretion and membrane function. PKCs were originally identified as serine/threonine protein kinases whose activity was dependent on calcium and phospholipids. Diacylglycerols (DAG) and tumor promoting phorbol esters bind to and activate PKC. PKCs can be subdivided into at least two major classes, including conventional (c) PKC isoforms (°™, æI, æII and Á) and novel (n) PKC isoforms ( 79oΩL, , 79oΩÊTO, , , l/i, m and n). Patterns of expression for each PKC isoform differ among tissues and PKC family members exhibit clear differences in their cofactor dependencies. For instance, the kinase activities of PKC 79oΩL and are independent of Ca2+. On the other hand, most of the other PKC members possess phorbol ester-binding activities and kinase activities.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa100-300) of human PRKCI protein (exact sequence is proprietary)

Isotype: IgG2b, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for PKC iota

Target: PKC iota (PRKCI) (Protein Kinase C, iota (PRKCI))

Alternative Name: Protein Kinase C iota

Background:

aPKC lambda/iota, Atypical protein kinase C lambda/iota, Atypical protein kinase C-lambda/iota, nPKC-iota, nPKCiota, PKC lambda/iota, PKCI, PKCiota, PKClambda, PRKC iota, PRKC lambda/iota, PRKCI, Protein kinase C iota type

Brain, Liver or placenta.

Molecular Weight: 68kDa

Gene ID: 5584, 478199

UniProt: P41743

Pathways: Neurotrophin Signaling Pathway, Cell-Cell Junction Organization, Tube Formation