FASL antibody (AA 107-222)

Catalog No. ABIN7863003

The Mouse Monoclonal anti-FASL antibody (Clone FASLG-4453) (ABIN7863003) specifically detects FASL in IHC. The antibody is reactive with Human samples.

Quick Overview for FASL antibody (AA 107-222) (ABIN7863003)

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This FASL antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: FASLG-4453

Product Details anti-FASL Antibody

Binding Specificity: AA 107-222

Purpose: Fas Ligand (FASLG) Antibody

Specificity: Cytotoxic T lymphocyte (CTL)-mediated cytotoxicity constitutes an importantcomponent of specific effector mechanisms in immuno-surveillance againstvirus-infected or transformed cells. Two mechanisms appear to account forthis activity, one of which is the perforin-based process. Independently, aFAS-based mechanism involves the transducing molecule FAS (also designated Apo-1) and its ligand (FAS-L). The human FAS protein is a cell surfaceglycoprotein that belongs to a family of receptors that includes CD40, nervegrowth factor receptors and tumor necrosis factor receptors. The FAS antigen is expressed on a broad range of lymphoid cell lines, certain of whichundergo apoptosis in response to treatment with antibody to FAS. Thesefindings strongly imply that targeted cell death is potentially mediated by the intercellular interactions of FAS with its ligand or effectors, and that FASmay be critically involved in CTL-mediated cytotoxicity.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Human recombinant FASLG protein fragment (around aa107-222) (exact sequence is proprietary)

Isotype: IgG1, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Expiry Date: 24 months

Target Details for FASL

Target: FASL (Fas Ligand (TNF Superfamily, Member 6) (FASL))

Alternative Name: Fas Ligand

Background:

ALPS1B, Apoptosis (APO 1) antigen ligand 1, APT1LG1, CD178 antigen, CD95 ligand protein,Generalized lymphoproliferative disease (Gld), soluble form, Tumor necrosis factor ligand superfamily member 6 (TNFL6 / TNFSF6)

Human tonsil or prostate.

Molecular Weight: 26kDa (soluble FAS-L) / 40kDa (FAS-L membrane)

Gene ID: 356, 2007

UniProt: P48023

Pathways: Apoptosis, EGFR Signaling Pathway, Production of Molecular Mediator of Immune Response, Positive Regulation of Endopeptidase Activity