Recombinant Dystrophin antibody (AA 1700-2300)

Catalog No. ABIN7864334

The Rabbit Monoclonal anti-Dystrophin antibody is suitable to detect Dystrophin in samples from Human. It has been validated for IHC, WB and IF.

Quick Overview for Recombinant Dystrophin antibody (AA 1700-2300) (ABIN7864334)

Target: Dystrophin (DMD)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Dystrophin antibody is un-conjugated

Application: Immunohistochemistry (IHC), Western Blotting (WB), Immunofluorescence (IF)

Clone: DMD-8773R

Product Details anti-Dystrophin Antibody

Binding Specificity: AA 1700-2300

Purpose: Recombinant Dystrophin (DMD) (Marker of Duchenne and Becker Muscular Dystrophy) Antibody

Specificity: Dystrophin-glycoprotein complex (DGC) connects the F-Actin cytoskeleton on the inner surface of muscle fibers to the surrounding extracellular matrix, through the cell membrane interface. A deficiency in this protein contributes to Duchenne (DMD) and Becker (BMD) muscular dystrophies. The human dystrophin gene measures 2.4 megabases, has more than 80 exons, produces a 14 kb mRNA and contains at least 8 independent tissue-specific promoters and 2 poly A sites. The dystrophin mRNA can undergo differential splicing and produce a range of transcripts that encode a large set of proteins. Dystrophin represents approximately 0.002 % of total striated muscle protein and localizes to triadic junctions in skeletal muscle, where it is thought to influence calcium ion homeostasis and force transmission.

Purification: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G.

Immunogen: Recombinant fragment (around aa1700-2300) of human DMD (exact sequence is proprietary).

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Concentration: 200 μg/mL

Buffer: Prepared in 10 mM PBS with 0.05 % BSA & 0.05 % azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Antibody with azide - store at 2 to 8°C. Antibody without azide - store at -20 to -80°C.Antibody is stable for 24 months. Non-hazardous. No MSDS required.

Target Details for Dystrophin

Target: Dystrophin (DMD)

Alternative Name: Dystrophin

Background:

BMD, CMD3B, Duchenne muscular dystrophy (DMD), Dystrophin, Muscular dystrophy Duchenne and Becker types

Human skeletal muscle and heart muscle tissues (IHC).

Molecular Weight: 427kDa

Gene ID: 1756, 495912

UniProt: P11532

Pathways: Skeletal Muscle Fiber Development